Notice of Application Notice is given that application has been made to the Office of the Comptroller of the Currency (the "OCC"), located at 425 South Financial Place, Suite 1700, Chicago, IL 60605, for consent to merge MidWestOne Bank ("MidWestOne"), main office located in Iowa City, Iowa 52240, with and into Nicolet National Bank ("Nicolet"), main office located in Green Bay, Wisconsin 54301 (the Nicolet- MidWestOne Merger). This notice is published pursuant to 12 USC 1828(c) and 12 CFR 5. Anyone may submit written comments on this application within 30 days after first publication of this notice to: Director of District Licensing 425 South Financial Place, Suite 1700 Chicago, IL 60605 (312) 360-8863 Licensing@occ.treas.gov The public may find information regarding this application, including the date of the end of the public comment period, in the OCC Weekly Bulletin at www.occ.gov. Requests for a copy of the public file on the application should be made to the Director of District Licensing. Dated: November 24, 2025 MidWestOne Bank, Iowa City, Iowa Nicolet National Bank, Green Bay, Wisconsin November 24, December 1 2025 LIOW0409869